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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: BRCA1 Localization to the Telomere and Its Loss from the Telomere in Response to DNA Damage
doi: 10.1074/jbc.M109.025825
Figure Lengend Snippet: Colocalization of BRCA1 with TRF1 and TRF2. Subconfluent proliferating T47D cells were sham-treated or exposed to ADR (7 μm) for 8 h and processed for confocal microscopy. Images are shown of DAPI stain (nuclei), BRCA1 immunostaining (green), TRF1 or TRF2 immunostaining (red), and merged BRCA1 plus TRF1 (A) or BRCA1 plus TRF2 (B) images. Yellow granules in the merged images indicate regions of colocalization. Quantitation of colocalization of BRCA1/TRF1 and BRCA1/TRF2 was carried out using Metamorph version 6.2 software (n = 25 cells).
Article Snippet: Quantitation of colocalization of BRCA1/TRF1 and BRCA1/TRF2 was carried out using
Techniques: Confocal Microscopy, Staining, Immunostaining, Quantitation Assay, Software
Journal: The Journal of Biological Chemistry
Article Title: BRCA1 Localization to the Telomere and Its Loss from the Telomere in Response to DNA Damage
doi: 10.1074/jbc.M109.025825
Figure Lengend Snippet: Teli-FISH of BRCA1 colocalization with telomeric probe. Subconfluent proliferating T47D cells on slides were fixed and hybridized to a Cy3-labeled telomere-specific PNA probe. The slides were immunostained with BRCA1, TRF1, or TRF2 primary antibody and secondary antibody conjugated to Alexa Fluor dye and processed for confocal imaging. Images are shown of DAPI stain (blue), PNA probe (red), BRCA1 (green), TRF1 (green), or TRF2 (green). Quantification of colocalization of BRCA1, TRF1, or TRF2 with the PNA probe (yellow granules in merged images) was carried out using Metamorph version 6.2 software (n = 28) or by manual counting of the percentage of total telomeric granules (yellow + red) that is colocalized (yellow) with BRCA1, TRF1, or TRF2 (n = 3 cells).
Article Snippet: Quantitation of colocalization of BRCA1/TRF1 and BRCA1/TRF2 was carried out using
Techniques: Labeling, Imaging, Staining, Software